Maria Luisa Mangoni
Associate Professor, University of Rome
Derivatives of the Frog-Skin Peptide Esculentin-1a with Promising activity Against Infections Induced by Pseudomonas Aeruginosa
Maria Luisa Mangoni (1), Alison M. McDermott (2), Y. Peter Di (3)
(1)Laboratory affiliated to Istituto Pasteur-Italia Fondazione Cenci Bolognetti, Department of Biochemical Sciences, Sapienza University of Rome, ITALY; (2)College of Optometry, University of Houston, Houston, USA; (3)Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, USA
Pseudomonas aeruginosa is an opportunistic pathogen causing a variety of infections including pneumonia in cystic fibrosis sufferers; microbial keratitis in contact lens wearers as well as infected skin wounds. We investigated the anti-pseudomonal efficacy of a frog skin-derived AMP, Esculentin-1a(1-21)NH2, [Esc(1-21)]. Our results revealed that it has (i) rapid killing kinetics against both free-living and biofilm forms of this pathogen, with a membrane-perturbing activity as a plausible mode of action limiting the emergence of resistance; (ii) the capability to preserve its bactericidal activity in physiological environments; (iii) the ability to induce migration of bronchial epithelial cells and presumably to accelerate the recovery of an injured bronchial epithelium without being cytotoxic. Overall, Esc(1-21) and its derivatives represent attractive alternatives for local treatment of Pseudomonas-associated infections.
This work was supported by grants from Sapienza University of Rome; the Italian Cystic Fibrosis Research Foundation (Project FFC#11/2014); NIH (EY13175; EY07551)