Scientist, Roche Madison
Massively Parallel Synthesis and Screening of Linear Peptides and Macrocycles using Peptide Microarrays
We have developed a peptide synthesis and screening platform for the discovery of peptidic molecules that bind to protein targets of interest. This technique employs the use of digital micromirror devices to direct the maskless synthesis of millions of unique peptides in parallel on a microarray surface. Using a chemical catalog of over 300 amino acid building blocks (natural and non-natural), we can synthesize 18 million unique peptides in a 24-48 hour run. The method can be applied to synthesize both linear and cyclic peptidic molecules, enabling screens in a diverse chemical space. Here, we will describe the core technology and its application to rapidly and systematically evolve high-affinity, high-specificity binding peptides to protein targets in a reproducible and digitally controlled process.