VP Preclinical Development, Magenta Therapeutics
Analytical & Functionally Comparison of a Synthetic vs Recombinant Chemokine
Chemokines are small peptide ligands (8 – 10 KDa) that bind to G-protein coupled receptors. Once bound to their receptor, chemokines elicit a variety of intracellular signaling pathways to regulate diverse biological functions including chemotaxis, inflammation and angiogenesis. There are four major classes of chemokines called CXC, CC, CXC3, and XC based on the spacing between cysteines. The manufacturing of chemokines is typically done using recombinant DNA engineering, expression in E. Coli, followed by peptide isolation and refolding. We describe here the functional and analytical characterization of a synthetically generated chemokine compared to a bacterially expressed, recombinant form of the same peptide.