The ARGEN, by Fluence Analytics, provides researchers the tools to quickly detect changes in the normalized molecular weight of peptide samples in solution, enabling them to identify the onset of aggregation, degradation or phase changes while these phenomena take place. The ARGEN does this by continuously monitoring multiple independent static light scattering experiments, each one with its own set of controlled stressor conditions. These ARGEN controlled stressor conditions include both temperature from 18 oC – 100 oC as well as stirring from 0 – 2000 RPM but researchers can additionally screen many common formulation conditions for each sample by adjusting the solvent, pH, salt type and concentration and excipient type and concentration.
The data presented, shows how ARGEN was used to study the stability of peptides as influenced by pH, sample concentration, temperature and stirring stress in real time. This information can be used to preemptively understand how and when aggregation or degradation may occur so that additional formulation development can be focused to minimize these unwanted results.