David, Feldan Therapeutics
Novel peptide technology to deliver proteins and CRISPR ribonucleoproteins in cells
Viral and non-viral delivery methods that introduce foreign genetic material in cells are widely used in cell therapy applications. While effective in certain cell types, current techniques often are detrimental to cell viability and cell potency, leave genetic footprints, and pose important safety and regulatory concerns. One promising avenue to bypass the use of foreign DNA and virus in cell therapy consists on the direct delivery of active proteins. However, the poor efficiency of current protein delivery methods, mainly caused by endosomal entrapment, slowed down the transfer of this approach toward cell therapy.
In order to fulfill a need for an efficient and appropriate protein delivery method for ex vivo cell therapy and in vivo administration through local injections, we developed a proprietary peptide technology that enables robust entry of fluorescent proteins, antibodies, peptides and CRISPR nucleases in cells by a simple co-incubation. Initially based on natural domains, these carrier peptides that combine a cell penetration function with an endosomal leakage activity are now rationally designed and optimized for their ability to deliver proteins in hard-to-transfect cells, including Natural Killer cells and primary airway epithelia cells. The flexibility, efficiency and simplicity of this peptide-based technology, the Feldan Shuttle, open new avenues for ex vivo and in vivo therapies.
David Guay is the Research Director at Feldan Therapeutics since 2009. Dr. Guay received his PhD in molecular and cell biology from Laval University for its research on the implication of a transcription factor in DNA repair and chemotherapy resistance mechanisms. He holds a postdoctoral fellowship in chemical engineering from McGill University where he developed a DNA delivery technique based on a non-thermal plasma technology. His research at Feldan Therapeutics focuses on the “Feldan Shuttle”, a peptide-based technology designed to deliver native proteins, namely transcription factors, antibodies and CRISPR nucleases, in therapeutic cells.