BPS 2023
Toward an all – in – one purification and synthesis solution: PEC – grade peptides for fast and reliable drug development
Chloe Mitchell
Field Application Scientist, Gyros Protein Technologies
ABSTRACT
Purity no longer has to be at odds with speed and efficiency in early-stage drug development. Here we present the peptide synthesizer PurePep® Chorus and PurePep® Easy Clean (PEC) purification technology to address challenges faced in manufacturing novel peptide drug targets. Our PEC technology utilizes a catch and release approach, whereby a linker is covalently attached during SPPS, immobilized onto agarose beads and released through a reduction reaction – yielding the purified PEC-grade peptide of interest to the user[1]. When PEC is combined with the PurePep Chorus, users can have a faster and more flexible workflow from start to finish. The Chorus offers users with capabilities including (1) independent induction heating on each RV, that has adjustable deprotection and coupling temperatures, (2) reduced priming volumes, as expensive and sensitive monomers can be loaded in a SingleShotTM amino acids vial, and (3) live UV monitoring that takes the guess work out of achieving effective deprotection. Parallel synthesis and purification workflows have the capability of being achieved in a single day, as the PEC Auto Kit integrates well onto the Chorus, automating the parallel purification of six peptides in just over five hours. PEC provides an alternative app roach to conventional HPLC, that can be labor intensive and have high solvent consumption. Binding assays have validated that PEC-grade peptides provide comparable results to HPLC-grade peptides[2]. Overall, we hope our PurePep solutions can be combined to reliably produce high quality peptides, on a shorter timescale. 1 Zitterbart et al., Chem. Sci.
2021, 12, 2389-2396 2 S. Putta et al., Structure 2022, 30, p. 1.
BIO
Chloe Mitchell completed her PhD at the University of Toronto under the supervisor of Dr. Charles Deber. She worked on designing transmembrane peptides that were targeted toward the protein-protein interface of multidrug resistance efflux pumps in a broad-spectrum of multidrug resistant bacteria. She joined GPT after graduation as a field application scientist and is now helping the peptide community to get the maximum efficiency out of their GPT instruments and PEC purification kits.