Associate Professor, University of Copenhagen
Novel Strategy for O-Glycosylation of Peptide Hormones
Peptide hormones and neuropeptides encompass a large class of biologically active peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure by binding to specific receptors and mediate intracellular signalling. During biosynthesis peptide hormones undergo extensive post translational modification (PTM) essential for activity including C-terminal amidation, phosphorylation and proprotein convertase (PC) processing. Here, we have used a novel strategy for exploring potential O-glycosylation of peptide hormones in mammalian endocrine tissues, cell lines and biofluids using sensitive mass spectrometry, and surprisingly we identified wide occurrence of O-glycans on peptide hormones. We identified O-glycans in the receptor binding domains of mammalian endogenous mature peptide hormones, and demonstrate that the O-glycans serve to modulate receptor signalling and peptide hormone stability in vivo.
Dr. Schjoldager is an assistant professor at the University of Copenhagen whose major focus is to explore the biological role of O-glycans and post-translational modifications in health and disease. She is currently working on projects focusing on O-glycans in peptide hormone biology, lipid metabolism, LDLR and related receptor function and protein stability.