Senior Research Scientist, CEM
Physical and Chemical Considerations in Microwave Assisted Peptide Synthesis: Method Development to Automated cGMP Peptide Production
To address the needs of the market, new cGMP methodology has been developed utilizing automation and microwave assisted heating. This work details mechanistic-based, innovative improvements to the chemical methodology of solid phase peptide synthesis and addresses high temperature considerations such as aspartimide formation, epimerization, and heat-sensitive linkers (ex 2Cl trt PS). Key features include: (1) enhanced methods for high purity peptide synthesis, (2) green methodology with a demonstrated 95% reduction in solvent consumption, and (3) rapid scale up from R&D to production with cGMP considerations.