Method development for the manufacture of therapeutic peptides remains a vital piece of the commercial peptide production process. An optimized solid-phase synthesis protocol can be challenging to develop. Recent advances in peptide therapeutics focus on greater structural complexity making peptides that are more physiologically stable products with increased target specificity and membrane permeability . Peptides are also key players in the personalized medicine sector, which relies on faster peptide synthesis protocols and reduced processing times to reach patients effectively.
Here we show the complete parallel synthesis, from swelling to cleavage, of several biologically relevant peptides under different conditions, including increased temperatures (50-90°C), on an automated peptide synthesizer in high purity. In some examples, real-time UV deprotection monitoring has been used to assist in the synthesis optimization process. Optimization has also allowed a reduction in total synthesis times for preparation of the pure peptides.
 S. H. Joo, “Cyclic peptides as therapeutic agents and biochemical tools,” Biomol. Ther., 20, 1,19-26, 2012.