A considerable quantity of an alkylation by-product is observed when using 3,6-dioxa-1,8-octanedithiol as a scavenger during acidic release of peptides containing the thioether amino acid methionine from the solid support. Adjustment of the cleavage conditions by replacement of 3,6-dioxa-1,8-octanedithiol with ethane dithiol or by using methionine sulfoxide as an alternative to methionine resulted in no such impurity. The by-product was detectable by liquid chromatography and mass spectrometry and characterised by NMR spectroscopy of an isolated model peptide. It could be effectively removed in a separate post cleavage step by treatment with dilute aqueous acid at 37 °C. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.
We outline a novel alkylation when using the dithiol 3,6-dioxa-1,8-octanedithiol (DODT) as a scavenger in TFA-mediated peptide cleavage from the resin. Peptides containing unprotected methionine were extensively modified by a DODT-derived alkylating agent affording a previously unreported by-product. Conditions for the prevention or subsequent removal of the by-product are outlined.