Oligopeptides are of high importance for various industrial applications, e.g. cosmetical or medical. Homooligomerizations and co-oligomerizations with anionic amino acid esters are well described but a successful synthesis of cationic heterooligopeptides has been missing so far. The present study reports the ficain-catalyzed heterooligomerizations of LysOEt with MetOEt, leading to cationic heterooligopeptides with a yield up to 49.5% (w/w). MALDI-ToF/ToF-MS analyses proved successful syntheses of cationic heterooligopeptides with a DP between 7 and 10 amino acid residues, with the enzyme exhibiting a clear preference for methionine. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.
The present study reports the ficain-catalyzed heterooligomerizations of H-Lys-OEt with H-Met-OEt. MALDI-ToF/ToF-MS analyses proved successful syntheses of cationic heterooligopeptides with a degree of polymerization between 7 and 10 amino acid residues, with the enzyme exhibiting a clear preference for methionine.