Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

Chem Commun (Camb). 2015 Oct 14. [Epub ahead of print]

Cao Y¹, Nguyen GK, Tam JP, Liu CF.

Author information

¹School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.

Abstract

Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.